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To measure the rate of 89K excision, we developed a real-time quantitative PCR (qPCR) assay and established which the frequency of 89K excision from an 05ZYH33 overnight culture was ∼0.032%, a rate comparable to that observed for SLG excision in the Streptomyces lividans sixty six
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is preceded by a putative ribosome binding web page (AGGAG), which is located eight bp upstream in the initiation codon of xis
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To ascertain the frequency of 89K excision, real-time qPCR assays using SYBR Premix Ex Taq (TaKaRa) had been formulated to measure the percentage of cells inside a tradition that contained the 89K attP
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